Clinical Process Development Associate II - Molecular Biology

Broad Institute Platforms are dedicated to solving the world's most challenging biomedical problems through the continuous development and investment in cutting edge technology and large-scale research projects that are both challenging and complex.

In support of continually changing initiatives, the Clinical Laboratory Process Development Associate II is committed to carrying out innovative, high-throughput lab processes and supporting the highest quality research to understand the impacts of genetic regulation on human health and disease. In this role, you are responsible for processing samples through a high throughput clinical research laboratory and meeting production goals.

In this position, you will utilize a range of molecular biology methods (e.g. Gateway, GoldenGate, Gibson, In-fusion, and restriction/ligation cloning etc) to create transfection-quality DNA preps of genetic perturbation libraries (CRISPR, ORF, shRNA, Saturation Mutagenesis, and other perturbation types), as well as sample prep for next-gen sequencing (NGS), and glycerol/DNA/virus sample renewal/inventory management. In addition to the day-to-day efficient execution of molecular cloning projects, responsibilities include evaluating and enhancing the efficiency of lab processes, adaptation of new technologies, troubleshooting efforts, and participation in continuous improvement solutions through appropriate change management procedures.

The individual may be involved with the designing and executing of development, process improvement, or troubleshooting projects under direct guidance from the supervisor in support of platform goals and may be required to complete related training in JMP, Six Sigma, or other process improvement methods. Upon completion of these projects the individual is responsible for documenting and communicating results to others during dedicated meetings. In addition, PDAs will assist with the general supervision of work of other laboratory personnel such as Process Development Technicians and Accessioning Technicians as required.

Job Responsibilities:

* Produces genetic perturbation libraries with emphasis on efficiency, quality, precision. This includes vigorous lab process control and quality monitoring, and persistent focus on strict lab practices to minimize sample mishandling and contamination.

* Executes day-to-day operation in transfection-quality DNA prep workflow, including DNA prep, quantification, normalization, DNA quality monitoring, and the inventory management of the glycerol working copy and DNA products. Handles custom rearraying of the layout of glycerol and DNA samples.

* Follow standard operating procedures.

* Performs only those high complexity tests that are authorized by the Laboratory Director and require a degree of skill commensurate with the individual's education, training, experience, and technical abilities.

* Tests will require the exercise of independent judgment and responsibility with minimal supervision by the laboratory director or supervisor.

* Adhere to the laboratory's quality control policies, document all quality control activities, instrument and procedural calibrations and maintenance performed.

* Follow the laboratory's established policies and procedures whenever test systems are not within the laboratory's established acceptable levels of performance.

* Be capable of identifying problems that may adversely affect test performance or reporting of test results. - Correct the problems according to procedures or immediately notify the General Supervisor, Technical Supervisor, Clinical Consultant or Laboratory Director.

* Document all corrective actions taken when test systems deviate from the laboratory's established performance specifications.

* Review and monitor quality metrics and communicate results to others during dedicated meetings as needed.

Requirements:

* BS/BA degree in Biology or Chemistry. Please be prepared to provide a copy of the diploma and transcripts upon request.

* 2+ years of relevant full time laboratory experience subsequent to obtaining B.S./B.A. degree required.

* Working, solid knowledge of primary biological science principles required.

* Recent (within the last year) laboratory experience with the use of standard lab equipment such as pipettes and centrifuges required.

* Experience with automation preferred.

* Hands-on experience with genomic, microbiology, cell biology, and/or molecular biology laboratory methods preferred.

* Strong analytical skills, problem solving ability, and innovation aptitude required.

* Some experience with statistical analysis is preferred.

* Working knowledge of computers, databases, and working in a Windows/Mac environment required.

* Must have strong attention to detail, the ability to multi-task and strong organizational skills.

* Must follow Standard Operating Procedures and policies.

* Excellent written and oral communication skills, interpersonal skills and ability to effectively work as part of a team required.

* Ability to work independently with minimal supervision.

* Must thrive in a fast-paced environment.

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EOE / Minorities / Females / Protected Veterans / Disabilities
All Broad employees, regardless of work location, must be fully vaccinated for COVID-19 by Tuesday, October 12, 2021. Requests for exemption for medical or sincerely held religious beliefs will be considered.

The expected base pay range for this position as listed above is based on a 40 hour per week schedule. Broad provides pay ranges representing its reasonable and good faith estimate of what the organization reasonably expects to pay for a position at the time of posting. Actual compensation will vary based on factors including but not limited to, relevant skills, experience, education, qualifications, and other factors permissible by law.

At Broad, your base pay is just one part of a comprehensive total rewards package. From day one, this role offers a competitive benefits package including medical, dental, vision, life, and disability insurance; a 401(k) retirement plan; flexible spending and health savings accounts; at least 13 paid holidays; winter closure; paid time off; parental and family care leave; and an employee assistance program, among other Broad benefits.

The Broad Institute is an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, national origin, religion, age, color, sex, disability, protected veteran status, or any other characteristic protected by local, state, or federal laws, rules, or regulations.

Should you need a reasonable accommodation to complete the application or interview process, please contact recruiting@broadinstitute.org for assistance.