Research Lab Tech I

*Primary responsibilities include maintenance of human iPSC cultures, neuronal differentiation, and preparation of cells for drug treatment assays and downstream analysis, with a major focus on western blot and related biochemical assays.

*Plans and organizes experiments with long timelines, often requiring setup weeks to months in advance; success in this role requires strong organizational skills, attention to detail, and ability to anticipate experimental needs.

*Performs experimental work related to small-molecule treatments, phenotypic assays (e.g., viability assays, immunocytochemistry), and data collection.

*May occasionally assist with the processing of animal or human brain tissue for protein or RNA extraction and downstream molecular analysis.

*Reads and follows experimental protocols; after training, independently plans weekly schedule and executes assigned experiments with minimal supervision.

*Identifies and communicates issues during experiments; participates in troubleshooting discussions with the PI and lab team.

*Adapts protocols as needed to improve experimental outcomes or address specific project needs.

*Analyzes experimental results, prepares statistical and graphical summaries, and contributes to data interpretation.

*Maintains accurate and up-to-date electronic lab notebooks, protocols, and inventory records.

*Prepares written and oral reports for internal meetings; assists in the preparation of figures, presentations, manuscripts, and grant materials.

*Performs literature searches to support experimental design, data interpretation, and troubleshooting.

*Prepares laboratory reagents and solutions; assists with ordering supplies and maintaining stock of chemicals and materials.

*Contributes to general lab maintenance and organization, including shared equipment care and workspace cleanliness.

*Research experience, ideally involving cell culture and/or human neuronal cell lines and hands-on experience with core molecular biology techniques.

*Proficiency in basic laboratory math required for molecular biology, such as calculating molarity, preparing dilutions, and generating or interpreting standard curves.

*After training, able to work independently by following detailed protocols and personal notes, execute experiments accurately. Also collaborates effectively as part of a team.

*Strong organizational skills with the ability to plan and manage an experimental schedule to maximize productivity.

*Excellent attention to detail and capacity to multitask across projects and timelines.

*Effective communication and interpersonal skills.

*Computer literacy and proficiency in data entry, electronic documentation, and basic data analysis and graphic visualization (e.g., Excel, GraphPad Prism).

Additional Preferred Qualifications (not required):

*Hands-on experience with western blot (priority), qRT-PCR, molecular cloning, CRISPR/Cas9 editing.

*Understanding of experimental theory and ability to function with precision.

*Proficiency in software used for data analysis and presentation (e.g., Microsoft Excel, PowerPoint, Word, GraphPad Prism, Adobe Photoshop or Image J).

Minimum of HighSchoolDiplomaorEquivalentrequired

Bachelor'sDegree required (Biochemistry, Molecular Biology or related scientific field preferred)

Priorlaboratoryexperience,eitherthroughacademiccoursework,internships,oremployment.1-2yearspreferred

• Prior experience with neuronal cell culture and biochemical techniques such as western blotting are a plus.
• Familiarity with other molecular biology methods (e.g., PCR, cloning, CRISPR editing) is also highly desirable. Experience with animal work-such as brain dissections and tissue processing for protein or RNA analysis-will be highly regarded.

Research laboratory with typical hazards including chemicals, fumes and sharp instruments. Periods of prolonged standing and computer work may occur as well as other physical requirements such as walking.This role may occasionally require flexible scheduling, including rare weekend work, to support ongoing cell culture maintenance or time-sensitive experiments.

Prudent use of hospital resources.Cost-effective ordering of supplies.